GSK3B Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P49841 |
| Gene Id | 2932 |
| Gene Symbols | GSK3B |
| Protein Name / Synonyms | GSK3B, glycogen synthase kinase-3 beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
46kDa/48kDa
Observed MW
47kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GSK3B (NP_001139628.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GSK3B antibody (144-06164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot analysis of extracts from normal (control) and GSK3B knockout (KO) HeLa cells, using GSK3B antibody (144-06164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using GSK3B antibody (144-06164) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GSK3B antibody (144-06164) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using GSK3B antibody (144-06164) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using GSK3B antibody (144-06164) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of HeLa cells using GSK3B antibody (144-06164) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug GSK3B antibody (144-06164). Western blot was performed from the immunoprecipitate using GSK3B antibody (144-06164) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.