[KO Validated] GSS Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P48637 |
| Gene Id | 2937 |
| Gene Symbols | GSS |
| Protein Name / Synonyms | GSS, GSHS, HEL-S-64p, HEL-S-88n, glutathione synthetase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
40kDa/52kDa
Observed MW
54kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GSS antibody (144-60698) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.![Western blot - [KO Validated] GSS Polyclonal Antibody (144-60698)](https://cdn.sanity.io/images/g51potcz/production/256a76b302e99c4d064838108bcd48b90ad3428c-345x350.jpg)
Western blot analysis of extracts from normal (control) and GSS knockout (KO) 293T cells, using GSS antibody (144-60698) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] GSS Polyclonal Antibody (144-60698)](https://www.raybiotech.com/files/images/Antibodies/144/144-60698/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.