The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
38kDa/41kDa
Observed MW
41kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF3A (NP_002088.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of SW480 cells, using GTF3A antibody (144-08426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Expiration:
12 months from the date of shipment when stored properly.
