WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 22-115 of human GUCA2A (NP_291031.2).
Western blot analysis of extracts of various cell lines, using GUCA2A antibody (144-09820) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 5s.
12 months from the date of shipment when stored properly.