HADH Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16836 |
| Gene Id | 3033 |
| Gene Symbols | HADH |
| Protein Name / Synonyms | HADH, HAD, HADH1, HADHSC, HCDH, HHF4, MSCHAD, SCHAD, hydroxyacyl-CoA dehydrogenase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
34kDa/36kDa/42kDa
Observed MW
34kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human HADH (NP_005318.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HADH antibody (144-01076) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat liver using HADH antibody (144-01076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HADH antibody (144-01076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HADH antibody (144-01076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spinal cord using HADH antibody (144-01076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HADH antibody (144-01076) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HADH antibody (144-01076) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.