HADHA Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P40939 |
| Gene Id | 3030 |
| Gene Symbols | HADHA |
| Protein Name / Synonyms | HADHA, ECHA, GBP, HADH, LCEH, LCHAD, MTPA, TP-ALPHA, trifunctional enzyme subunit alpha, mitochondrial |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
28kDa/83kDa
Observed MW
82kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HADHA antibody (144-05346) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.![Western blot - [KO Validated] HADHA Polyclonal Antibody (144-05346)](https://cdn.sanity.io/images/g51potcz/production/83cd960cde57abb4d13c7a389f003d34f0d9d1b7-536x350.jpg)
Western blot analysis of extracts of various cells, using HADHA antibody (144-05346) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.![Western blot - [KO Validated] HADHA Polyclonal Antibody (144-05346)](https://cdn.sanity.io/images/g51potcz/production/a7064af0e1bc9e7ad0b850eae28d902040167fa2-367x550.jpg)
Western blot analysis of extracts from normal (control) and HADHA knockout (KO) 293T cells, using HADHA antibody (144-05346) at 1:1000 dilution.![Western blot - [KO Validated] HADHA Polyclonal Antibody (144-05346)](https://www.raybiotech.com/files/images/Antibodies/144/144-05346/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.