HDAC6 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UBN7 |
| Gene Id | 10013 |
| Gene Symbols | HDAC6 |
| Protein Name / Synonyms | HDAC6, CPBHM, HD6, JM21, PPP1R90, histone deacetylase 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
114kDa/131kDa
Observed MW
170kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HDAC6 antibody (144-11259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using HDAC6 antibody (144-11259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (144-11259). Western blot was performed from the immunoprecipitate using HDAC6 antibody (144-11259) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.