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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P51858 |
| Gene Id | 3068 |
| Gene Symbols | HDGF |
| Protein Name / Synonyms | HDGF, HMG1L2, heparin binding growth factor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
25kDa/26kDa/28kDa
Observed MW
38kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HDGF antibody (144-05347) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HDGF Antibody (144-05347) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.