Rabbit anti-Heat shock protein 1 (HSPE1) Biotinylated Antibody, 50 ug
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | AAH23518 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:32, 000), Western Blotting (recommended work dilution= 1: 1, 000-5, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant proteinHSP10. The membrane blot was probed with anti-HSP10 primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant proteinHSP10 from E.coli cell (12kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Protein Folding |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Heat shock protein 10(HSP10) is a protein can form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner encoded by a nuclear gene HSPE1.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat HSP10 has not been tested yet.
Related Products
Recombinant Heat Shock Protein 10 (HSP10) /GroES (Cat: 228-10581-3) Recombinant E. coli Hsp10 / GroES (Cat: 228-10581-1)
References
- Anna C. Kayani, et al. Overexpression of HSP10 in skeletal muscle of transgenic mice prevents the agerelated fall in maximum tetanic force generation and muscle cross-sectional area. Am J Physiol Regul Integr Comp Physiol. 2010 July; 299(1): R268R276.
- Haibo Jia, et al. Heat shock protein 10 (Hsp10) in immune-related diseases: one coin, two sides. Int J Biochem Mol Biol. 2011; 2(1): 4757.
Expiration:
12 months from the date of shipment when stored properly.
