HFM1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | A2PYH4 |
| Gene Id | 164045 |
| Gene Symbols | HFM1 |
| Protein Name / Synonyms | HFM1, MER3, POF9, SEC63D1, Si-11, Si-11-6, helicase, probable ATP-dependent DNA helicase HFM1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is thought to be an ATP-dependent DNA helicase and is expressed mainly in germ-line cells. Defects in this gene are a cause of premature ovarian failure 9 (POF9).
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
62kDa/162kDa
Observed MW
163kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HFM1 (NP_001017975.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of K-562 cells, using HFM1 antibody (144-08600) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Expiration:
12 months from the date of shipment when stored properly.