Rabbit Anti-HIF1A (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Hamster |
| Host Species | Rabbit |
| Accession Number | Q16665 |
| Gene Id | 3091 |
| Gene Symbols | HIF1A |
| Protein Name / Synonyms | HIF1A (N-term) |
| Clonality | Polyclonal |
| Preparation | This HIF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIF1A. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Angiogenesis, Inflammation, Autophagy, Hypoxia Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This protein encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.
Specificity
Predicted Reactivity
Bovine
Other Names
HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; Member of PAS protein 1; PAS domain-containing protein 8
NCBI Accession #
NP_001230013.1;NP_001521.1;NP_851397.1
Other Accession #
Q9XTA5
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
92670
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
 | Western blot analysis of HIF1A Antibody (N-term) (Cat# 102-15759) in CHO cell line lysates (35ug/lane). HIF1A (arrow) was detected using the purified Pab. |
 | HIF1A Antibody (N-term)(Cat# 102-15759) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIF1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Fluorescent confocal image of Hela cell stained with HIF1A Antibody (N-term)(Cat# 102-15759). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIF1A primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).HIF1A immunoreactivity is localized to cytoplasm and nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.