Rabbit Anti-HIF1Alpha (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q16665 |
| Gene Id | 3091 |
| Gene Symbols | HIF1A |
| Protein Name / Synonyms | HIF1Alpha (C-term) |
| Clonality | Polyclonal |
| Preparation | This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-757 amino acids from the C-terminal region of human HIF1Alpha. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF, IHC-P |
| Research Area | Angiogenesis, Cardiovascular Disease, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT).
Specificity
Other Names
HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; Member of PAS protein 1; PAS domain-containing protein 8
NCBI Accession #
NP_001230013.1;NP_001521.1;NP_851397.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
92670
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; IHC: 1:10-50
 | Western blot analysis of HIF1Alpha Antibody (C-term) (Cat# 102-17607) in MDA-MB231 cell line lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab. |
 | Western blot analysis of HIF1Alpha Antibody (C-term) (Cat# 102-17607) in mouse cerebellum tissue lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab. |
 | Confocal immunofluorescent analysis of HIF1Alpha Antibody (C-term)(Cat# 102-17607) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohis |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.