HIF1AN Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NWT6 |
| Gene Id | 55662 |
| Gene Symbols | HIF1AN |
| Protein Name / Synonyms | HIF1AN, FIH1, hypoxia-inducible factor 1-alpha inhibitor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
40kDa
Observed MW
40kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN (NP_060372.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HIF1AN antibody (144-05466) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using HIF1AN antibody (144-05466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HIF1AN antibody (144-05466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HIF1AN antibody (144-05466) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HIF1AN antibody (144-05466). Western blot was performed from the immunoprecipitate using HIF1AN antibody (144-05466) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.