HIRIP3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9BW71 |
| Gene Id | 8479 |
| Gene Symbols | HIRIP3 |
| Protein Name / Synonyms | HIRIP3, HIRA-interacting protein 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
19kDa/27kDa/61kDa
Observed MW
62kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (144-07014) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using HIRIP3 antibody (144-07014) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using HIRIP3 antibody (144-07014) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HIRIP3 antibody (144-07014) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using HIRIP3 antibody (144-07014) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody (144-07014) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using HIRIP3 antibody (144-07014) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HIRIP3 antibody (144-07014) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HIRIP3 antibody (144-07014) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HIRIP3 antibody (144-07014) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (144-07014).
Expiration:
12 months from the date of shipment when stored properly.