HIST2H2BE Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16778 |
| Gene Id | 8349 |
| Gene Symbols | HIST2H2BE |
| Protein Name / Synonyms | HIST2H2BE, GL105, H2B, H2B.1, H2BFQ, H2BGL105, H2BQ, histone H2B type 2-E |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
13kDa
Observed MW
15kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HIST2H2BE (NP_003519.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HIST2H2BE antibody (144-01958) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human esophagus using HIST2H2BE antibody (144-01958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using HIST2H2BE antibody (144-01958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HIST2H2BE antibody (144-01958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HIST2H2BE antibody (144-01958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HIST2H2BE antibody (144-01958) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.