HLA-E, HLA-6.2, QA1, EA1.2, EA2.1, MHC, major histocompatibility complex, class I, E
Accession Number
P13747
Gene Symbols
HLA-E
Gene Id
3133
Recommended Applications
Western Blotting
Purity
Affinity purification
Background
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
40kDa
Observed MW
45kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HLA-E (NP_005507.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HLA-E antibody (144-08429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.
