Rabbit Anti-HOMEZ (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q8IX15 |
| Gene Id | 57594 |
| Gene Symbols | HOMEZ |
| Protein Name / Synonyms | HOMEZ (N-term) |
| Clonality | Polyclonal |
| Preparation | This HOMEZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the N-terminal region of human HOMEZ. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
May function as a transcriptional regulator.
Specificity
Other Names
HOMEZ; KIAA1443; Homeobox and leucine zipper protein Homez; Homeodomain leucine zipper-containing factor
NCBI Accession #
NP_065885.2
Other Accession #
NP_065885.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
61240
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50
 | HOMEZ Antibody (N-term) (Cat# 102-10796) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow). |
 | HOMEZ Antibody (N-term) (Cat# 102-10796) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term)(Cat# 102-10796).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HOMEZ immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.