The protein encoded by this gene shares similarity with mouse Hs1bp3, an Hcls1/Hs1-interacting protein that may be involved in lymphocyte activation.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HS1BP3 (NP_071905.3).
Western blot analysis of extracts of various cell lines, using HS1BP3 antibody (144-08250) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human stomach using HS1BP3 antibody (144-08250) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HS1BP3 antibody (144-08250) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HS1BP3 antibody (144-08250) at dilution of 1:100. Blue: DAPI for nuclear staining.
12 months from the date of shipment when stored properly.