Rabbit Anti-HSF1 Sumoylation Site Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q00613 |
| Gene Id | 3297 |
| Gene Symbols | HSF1 |
| Protein Name / Synonyms | HSF1 Sumoylation Site |
| Clonality | Polyclonal |
| Preparation | This HSF1 Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-309 amino acids from human HSF1 Sumoylation Site. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cancer, Post-Translational Modifications |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.
Specificity
Predicted Reactivity
Bovine
Other Names
HSF1; HSTF1; Heat shock factor protein 1; Heat shock transcription factor 1
NCBI Accession #
NP_005517.1
Other Accession #
Q08DJ8
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
57260
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | HSF1 Antibody (Cat# 102-15039) western blot analysis in Jurkat cell line and mouse heart tissue lysates (35ug/lane).This demonstrates the HSF1 antibody detected the HSF1 protein (arrow). |
 | Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody (Cat# 102-15039) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.