WB 1:500 - 1:2000
IHC 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 474-643 of human HSPA6 (NP_002146.2).
Western blot analysis of extracts of various cell lines, using HSPA6 antibody (144-07688) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using HSPA6 antibody (144-07688) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HSPA6 antibody (144-07688) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.