HSPD1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P10809 |
| Gene Id | 3329 |
| Gene Symbols | HSPD1 |
| Protein Name / Synonyms | HSPD1, CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, 60 kDa heat shock protein, mitochondrial |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Apoptosis, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
17kDa/61kDa
Observed MW
61kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 27-240 of human HSPD1 (NP_002147.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HSPD1 antibody (144-00969) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot analysis of extracts of various cells, using HSPD1 antibody (144-00969) at 1:3000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat kidney using HSPD1 antibody (144-00969) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HSPD1 antibody (144-00969). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.