14-3-3 beta antibody [N1C2], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 7529 |
| Protein Name / Synonyms | 14-3-3 beta |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
GW128 antibody, HS1 antibody, KCIP-1 antibody, YWHAA antibody, YWHAB antibody, "brain protein 14-3-3, beta isoform antibody", protein 1054 antibody, 14-3-3 protein beta/alpha antibody, protein kinase C inhibitor protein-1 antibody, "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide antibody", 14-3-3 alpha antibody, protein kinase C inhibitor protein 1 antibody, "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
28 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 171 of 14-3-3 beta (Uniprot ID# P31946)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using 14-3-3 beta(107-11051) antibody at 1:500 dilution.
Sample (20 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-11051 diluted at 1:10000
A: mouse brain
12% SDS PAGE
107-11051 diluted at 1:10000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta (107-11051) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Hela
D: Hep G2
E: Molt-4
F: Raji
12% SDS PAGE
107-11051 diluted at 1:10000
A: A431
B: H1299
C: Hela
D: Hep G2
E: Molt-4
F: Raji
12% SDS PAGE
107-11051 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.