Rabbit Anti-Human ACF (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9NQ94 |
| Gene Id | 29974 |
| Gene Symbols | A1CF |
| Protein Name / Synonyms | ACF (C-term) |
| Clonality | Polyclonal |
| Preparation | This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human ACF. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.
Specificity
Other Names
A1CF; ACF; ASP; APOBEC1 complementation factor; APOBEC1-stimulating protein
NCBI Accession #
NP_001185747.1;NP_001185748.1;NP_001185749.1;NP_055391.2;NP_620310.1;NP_620311.1
Other Accession #
NP_620310
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
65202
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | ACF Antibody (C-term) (Cat# 102-10746) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ACF antibody detected the ACF protein (arrow). |
 | ACF Antibody (C-term) (Cat# 102-10746) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACF Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | ACF Antibody (C-term) (Cat# 102-10746) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.