ACTL8 antibody, 0.71 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 81569 |
| Protein Name / Synonyms | ACTL8 |
| Concentration (lot specific) | 0.71 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CT57 antibody, ACTL8 antibody, actin like protein antibody, actin-like protein 8 antibody, cancer/testis antigen 57 antibody, actin-like 8 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
41 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 156 and 366 of ACTL8 (Uniprot ID# Q9H568)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ACTL8(107-10640) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using ACTL8(107-10640) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
B: Molt-4
10% SDS PAGE
107-10640 diluted at 1:1000
A: Hep G2
B: Molt-4
10% SDS PAGE
107-10640 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.