AKR1B10 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 57016 |
| Protein Name / Synonyms | AKR1B10 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AKR1B11 antibody, AKR1B12 antibody, ALDRLn antibody, ARL-1 antibody, ARL1 antibody, HIS antibody, HSI antibody, MGC14103 antibody, AKR1B10 antibody, aldo-keto reductase family 1 member B10 antibody, hARP antibody, small intestine reductase antibody, aldose reductase-like peptide antibody, SI reductase antibody, aldose reductase-like 1 antibody, aldo-keto reductase family 1, member B11 (aldose reductase-like) antibody, ARP antibody, aldose reductase-related protein antibody, aldo-keto reductase family 1, member B10 (aldose reductase) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
36 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 316 of AKR1B10 (Uniprot ID# O60218)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
AKR1B10 antibody detects AKR1B10 protein by Western blot analysis.
A. 50 µg mouse colon lysate/extract
10 % SDS-PAGE
AKR1B10 antibody (107-10851) dilution: 1:5000
A. 50 µg mouse colon lysate/extract
10 % SDS-PAGE
AKR1B10 antibody (107-10851) dilution: 1:5000
AKR1B10 antibody detects AKR1B10 protein by Western blot analysis.
A. 50 µg rat colon lysate/extract
10 % SDS-PAGE
AKR1B10 antibody (107-10851) dilution: 1:5000
A. 50 µg rat colon lysate/extract
10 % SDS-PAGE
AKR1B10 antibody (107-10851) dilution: 1:5000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10(107-10851) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10(107-10851) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: MOLT4
B: Raji
10% SDS PAGE
107-10851 diluted at 1:1000
A: MOLT4
B: Raji
10% SDS PAGE
107-10851 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.