Rabbit Anti-Human AKT1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P31749 |
| Gene Id | 207 |
| Gene Symbols | AKT1 |
| Protein Name / Synonyms | AKT1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the N-terminal region of human AKT1. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Angiogenesis, Apoptosis, Autophagy, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Specificity
Predicted Reactivity
Mouse, Bovine
Other Names
AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; RAC-PK-alpha
NCBI Accession #
NP_001014431.1;NP_001014432.1;NP_005154.2
Other Accession #
P31750; Q01314
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55686
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of AKT1 Antibody (N-term) polyclonal antibody(Cat# 102-17145)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with AKT1 antibody (N-term)(Cat# 102-17145), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | AKT1 Antibody (N-term) (Cat# 102-17145) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow). |
 | Confocal immunofluorescent analysis of AKT1 Antibody (N-term)(Cat# 102-17145) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
 | AKT1 Antibody (N-term) (Cat# 102-17145) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.