Rabbit Anti-Human AKT2 (S474) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P31751 |
| Gene Id | 208 |
| Gene Symbols | AKT2 |
| Protein Name / Synonyms | AKT2 (S474) |
| Clonality | Polyclonal |
| Preparation | This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from human AKT2. |
| Concentration (lot specific) | 0.270 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cancer, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; RAC-PK-beta
NCBI Accession #
NP_001617.1
Other Accession #
P47197; Q60823
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55769
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; IHC: 1:10-50
 | Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (S474)(Cat# 102-17084).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).AKT2 immunoreactivity is localized to Cytoplasm significantly. |
 | Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.