Alpha-catulin antibody [N3C2], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 8727 |
| Protein Name / Synonyms | Alpha-catulin, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CLLP antibody, FLJ08121 antibody, alpha-CATU antibody, CTNNAL1 antibody, alpha-catulin antibody, alpha2-catulin antibody, catenin alpha-like protein 1 antibody, alpha-catenin-related protein antibody, ACRP antibody, "catenin (cadherin-associated protein), alpha-like 1 antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
82 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 292 and 563 of Alpha-catulin (Uniprot ID# Q9UBT7)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Alpha-catulin antibody [N3C2], Internal detects Alpha-catulin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Alpha-catulin protein stained by Alpha-catulin antibody [N3C2], Internal (107-11012) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Alpha-catulin protein stained by Alpha-catulin antibody [N3C2], Internal (107-11012) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-catulin(107-11012) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
7.5% SDS PAGE
107-11012 diluted at 1:1000
A: Hela
B: Hep G2
7.5% SDS PAGE
107-11012 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.