alpha Glucosidase II antibody [N1N2], N-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 23193 |
| Protein Name / Synonyms | alpha Glucosidase II, N-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
G2AN antibody, GLUII antibody, KIAA0088 antibody, GANAB antibody, alpha-glucosidase 2 antibody, glucosidase II subunit alpha antibody, neutral alpha-glucosidase AB antibody, alpha glucosidase II alpha subunit antibody, glucosidase, alpha, neutral AB antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
107 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 47 and 251 of alpha Glucosidase II
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Glucosidase II(107-10330) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10330 diluted at 1:1000
A: Mouse brain
7.5% SDS PAGE
107-10330 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using alpha Glucosidase II(107-10330) antibody at 1:500 dilution.
Sample(30 ug whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
7.5% SDS PAGE
107-10330 diluted at 1:1000
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
7.5% SDS PAGE
107-10330 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.