alpha Tubulin 1A antibody, 0.24 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 7846 |
| Protein Name / Synonyms | alpha Tubulin 1A |
| Concentration (lot specific) | 0.24 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
B-ALPHA-1 antibody, FLJ25113 antibody, LIS3 antibody, TUBA3 antibody, TUBA1A antibody, tubulin B-alpha-1 antibody, hum-a-tub1 antibody, "tubulin, alpha, brain-specific antibody", hum-a-tub2 antibody, tubulin alpha-1A chain antibody, "tubulin, alpha 3 antibody", alpha-tubulin 3 antibody, tubulin alpha-3 chain antibody, "tubulin, alpha 1a antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
50 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 227 and 440 (Q71U36) of alpha Tubulin 1A
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Breast ca, using alpha Tubulin 1A(107-11148) antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
107-11148 diluted at 1:1000
A: NIH-3T3
10% SDS PAGE
107-11148 diluted at 1:1000
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
alpha Tubulin 1A antibody (107-11148) dilution: 1:500.
Sample: Paraffin-embedded mouse ovary.
alpha Tubulin 1A antibody (107-11148) dilution: 1:500.
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
alpha Tubulin 1A antibody (107-11148) dilution: 1:500.
Sample: Paraffin-embedded mouse testis.
alpha Tubulin 1A antibody (107-11148) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Tubulin 1A(107-11148) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: JurKat
D: Raji
10% SDS PAGE
107-11148 diluted at 1:1000
A: 293T
B: A431
C: JurKat
D: Raji
10% SDS PAGE
107-11148 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.