Rabbit Anti-Human AMPK alpha (PRKAA1) (S487) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13131 |
| Gene Id | 5562 |
| Gene Symbols | PRKAA1 |
| Protein Name / Synonyms | AMPK alpha (PRKAA1) (S487) |
| Clonality | Polyclonal |
| Preparation | This AMPK alpha(PRKAA1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-502 amino acids from human AMPK alpha(PRKAA1). |
| Concentration (lot specific) | 0.310 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cancer, AMPK Signaling, Autophagy, Energy Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Specificity
Other Names
PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase; Tau-protein kinase PRKAA1
NCBI Accession #
NP_006242.5;NP_996790.3
Other Accession #
Q86VS1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
64009
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | Western blot analysis of PRKAA1 (arrow) using rabbit polyclonal PRKAA1 Antibody (S246) (Cat# 102-17219). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PRKAA1-pS487, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.