Rabbit Anti-Human Androgen Receptor Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P10275 |
| Gene Id | 367 |
| Gene Symbols | AR DHTR NR3C4 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was full recombinant protein derived from human androgen receptor. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 40, 000(at least detecting 25ng/ml)), Western Blotting (recommended work dilution= 1:1, 000-5, 000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.Androgen receptor protein ?g, Lane 2.MCF7 cell lysate , Lane 3.BSA 2?g. The membrane blot was probed with anti-Androgen receptor primary antibody(0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular androgen receptor from human cells (70-80 kDa). |
| Antigen Catalog Number | RB-14-0003P |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Apoptosis, Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
The human androgen receptor protein belongs to the class of nuclear receptors called activated class I steroid receptors, which also includes glucocorticoid receptor, progesterone receptor, and mineralocorticoid receptor. These receptors recognize canonical androgen response elements (AREs), which are inverted repeats of 5-prime-TGTTCT-3-prime. The major domains of AR include N- and Cterminal activation domains, which are designated activation function-1 (AF-1) and AF-2, a ligandbinding domain, and a polyglutamine tract. Human androgen receptor contain 920 amino acids and having a molecular mass of 110 kDa.
Typical Data
Specificity
The rabbit anti-human AR antibody detects specifically target derived from human recombinant protein as well as MCF7 cell lysate at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage/Stability
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
Expiration
12 months from the date of shipment when stored properly.
References
- J Trapman J, et al., Cloning, structure and expression of a cDNA encoding the human androgen receptor. Biochem Biophys Res Commun. 1988 May 1;153(1):241-8. PMID: 3377788
