AP1S2 antibody, 0.53 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 8905 |
| Protein Name / Synonyms | AP1S2 |
| Concentration (lot specific) | 0.53 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
MGC:1902 antibody, MRX59 antibody, SIGMA1B antibody, AP1S2 antibody, adapter-related protein complex 1 sigma-1B subunit antibody, sigma 1B subunit of AP-1 clathrin antibody, adaptor protein complex AP-1 sigma-1B subunit antibody, sigma1B-adaptin antibody, clathrin adaptor complex AP1 sigma 1B subunit antibody, golgi adaptor HA1/AP1 adaptin sigma 1B subunit antibody, sigma-adaptin 1B antibody, clathrin assembly protein complex 1 sigma-1B small chain antibody, golgi adaptor HA1/AP1 adaptin sigma-1B subunit antibody, AP-1 complex subunit sigma-2 antibody, "adaptor-related protein complex 1, sigma 2 subunit antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
19 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 157 of AP1S2 (Uniprot ID# P56377)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using AP1S2(107-10834) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
107-10834 diluted at 1:1000
A: Raji
12% SDS PAGE
107-10834 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.