Rabbit Anti-Human APG10L (C-term S116) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9H0Y0 |
| Gene Id | 83734 |
| Gene Symbols | ATG10 |
| Protein Name / Synonyms | APG10L (C-term S116) |
| Clonality | Polyclonal |
| Preparation | This APG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-121 amino acids from the C-terminal region of human APG10L. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
Specificity
Predicted Reactivity
Mouse
Other Names
ATG10; Ubiquitin-like-conjugating enzyme ATG10; Autophagy-related protein 10
NCBI Accession #
NP_001124500.1;NP_113670.1
Other Accession #
Q8R1P4; NP_001124500.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
25279
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | APG10L Antibody (C-term S116) (Cat# 102-12426) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow). |
 | Western blot analysis of APG10L (arrow) using rabbit polyclonal APG10L Antibody (C-term S116) (Cat# 102-12426). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG10L gene. |
 | APG10L Antibody (C-term S116) (Cat# 102-12426)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.