Rabbit Anti-Human APG7L(D555) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O95352 |
| Gene Id | 10533 |
| Gene Symbols | ATG7 |
| Protein Name / Synonyms | APG7L(D555) |
| Clonality | Polyclonal |
| Preparation | This APG7L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from human APG7L. |
| Concentration (lot specific) | 0.410 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
Specificity
Predicted Reactivity
Mouse, Rat, Chicken
Other Names
ATG7; APG7L; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; Ubiquitin-activating enzyme E1-like protein
NCBI Accession #
NP_001129503.2;NP_001138384.1;NP_006386.1
Other Accession #
Q641Y5; Q9D906; Q5ZKY2; NP_006386.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
77960
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | hAPG7L-D555 (PEI 1:100)b (Cat# 102-10983) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow). |
 | APG7L Antibody(D555) (Cat# 102-10983)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG7L Antibody(D555) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | APG7L Antibody (Cat# 102-10983) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.