Rabbit Anti-Human APRT (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P07741 |
| Gene Id | 353 |
| Gene Symbols | APRT |
| Protein Name / Synonyms | APRT (N-term) |
| Clonality | Polyclonal |
| Preparation | This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human APRT. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.
Specificity
Other Names
APRT; Adenine phosphoribosyltransferase
NCBI Accession #
NP_000476.1;NP_001025189.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
19608
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; IHC: 1:10-50; FC: 1:10-50
 | APRT Antibody (N-term) (Cat# 102-15210) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow). |
 | Fluorescent image of A549 cell stained with APRT Antibody (N-term)(Cat# 102-15210/SA091009AF).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.