ASNA1 antibody, 0.76 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 439 |
| Protein Name / Synonyms | ASNA1 |
| Concentration (lot specific) | 0.76 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ARSA-I antibody, ARSA1 antibody, GET3 antibody, MGC3821 antibody, TRC40 antibody, ASNA1 antibody, arsenical pump-driving ATPase antibody, golgi to ER traffic 3 homolog antibody, arsenite-stimulated ATPase antibody, ATPase ASNA1 antibody, "transmembrane domain recognition complex, 40kDa antibody", transmembrane domain recognition complex 40 kDa ATPase subunit antibody, "arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
39 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 45 and 279 of ASNA1 (Uniprot ID# O43681)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ASNA1(107-11120) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2500.
Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using ARSA(107-11120) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
107-11120 diluted at 1:1000
A: Mouse brain
10% SDS PAGE
107-11120 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
107-11120 diluted at 1:1000
A: HCT116
10% SDS PAGE
107-11120 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.