Rabbit Anti-Human ASPA (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P45381 |
| Gene Id | 443 |
| Gene Symbols | ASPA |
| Protein Name / Synonyms | ASPA (N-term) |
| Clonality | Polyclonal |
| Preparation | This ASPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human ASPA. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.
Specificity
Predicted Reactivity
Bovine
Other Names
ASPA; ACY2; ASP; Aspartoacylase; Aminoacylase-2
NCBI Accession #
NP_000040.1;NP_001121557.1
Other Accession #
P46446
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35735
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of ASPA Antibody (N-term) (Cat# 102-16976) in MCF7 cell line lysates (35ug/lane). ASPA (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of ASPA (arrow) using rabbit polyclonal ASPA Antibody (N-term) (Cat# 102-16976). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASPA gene. |
 | Confocal immunofluorescent analysis of ASPA Antibody (N-term)(Cat# 102-16976) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.