Synonyms
ASS antibody, CTLN1 antibody, ASS1 antibody, argininosuccinate synthetase 1 antibody, argininosuccinate synthase antibody, citrulline--aspartate ligase antibody, citrulline-aspartate ligase antibody, argininosuccinate synthase 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
47 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 198 of ASS1 (Uniprot ID# P00966)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

ASS1 antibody detects ASS1 protein by Western blot analysis.
A. 30 µg A431 whole cell lysate/extract
10 % SDS-PAGE
ASS1 antibody (107-10256) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ASS1(107-10256) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.