Synonyms
AICAR antibody, AICARFT antibody, FLJ93545 antibody, IMPCHASE antibody, PURH antibody, ATIC antibody, bifunctional purine biosynthesis protein PURH antibody, AICARFT/IMPCHASE antibody, phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase antibody, AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme antibody, 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase antibody, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
65 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 310 and 580 of ATIC (Uniprot ID# P31939)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Colon ca, using ATIC(107-10360) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATIC(107-10360) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
ATIC antibody
107-10360 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.