Rabbit Anti-Human ATP5O (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P48047 |
| Gene Id | 539 |
| Gene Symbols | ATP5O |
| Protein Name / Synonyms | ATP5O (N-term) |
| Clonality | Polyclonal |
| Preparation | This ATP5O antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-64 amino acids from the N-terminal region of human ATP5O. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
Specificity
Predicted Reactivity
Bovine, Pig
Other Names
ATP5O; ATPO; ATP synthase subunit O, mitochondrial; Oligomycin sensitivity conferral protein
NCBI Accession #
NP_001688.1
Other Accession #
Q2EN81; P13621
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
23277
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of ATP5O Antibody (N-term) (Cat# 102-17919) in 293 cell line lysates (35ug/lane). ATP5O (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI. |
 | Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of ATP5O (arrow) using rabbit polyclonal ATP5O Antibody (N-term) (Cat# 102-17919). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATP5O gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.