Rabbit Anti-Human B-RAF (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P15056 |
| Gene Id | 673 |
| Gene Symbols | BRAF |
| Protein Name / Synonyms | B-RAF (Center) |
| Clonality | Polyclonal |
| Preparation | This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from the Central region of human B-RAF. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | IF, FC, IHC-P, Western Blotting |
| Research Area | Cardiovascular Disease, Cancer, Ras/Rap Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
BRAF, a mebmer of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Specificity
Predicted Reactivity
Mouse, Chicken
Other Names
BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
NCBI Accession #
NP_004324.2
Other Accession #
P28028; Q04982
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
84437
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Fluorescent image of C2C12 cell stained with BRAF Antibody (Center)(Cat# 102-17635).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).BRAF immunoreactivity is localized to Cytoplasm significantly. |
 | Western blot analysis of BRAF Antibody (Center) polyclonal antibody(Cat# 102-17635)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human prostata carcinoma reacted with BRAF Antibody (Center)(Cat# 102-17635), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | B-RAF Antibody (Center) (Cat# 102-17635) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.