Rabbit Anti-Human BARON (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q92622 |
| Gene Id | 9711 |
| Gene Symbols | KIAA0226 |
| Protein Name / Synonyms | BARON (N-term) |
| Clonality | Polyclonal |
| Preparation | This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the N-terminal region of human BARON. |
| Concentration (lot specific) | 0.360 mg/ml |
| Recommended Applications | Western Blotting, IF, IHC-P, FC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Specificity
Other Names
KIAA0226; Run domain Beclin-1 interacting and cystein-rich containing protein; Beclin-1 associated RUN domain containing protein
NCBI Accession #
NP_001139114.1;NP_055502.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
108622
Recommended Dilutions
WB: 1:1000; IF: 1:100; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of BARON antibody (N-term) (Cat# 102-13787) in MCF-7 cell line lysates (35ug/lane). BARON (arrow) was detected using the purified Pab. |
 | Fluorescent image of U251 cells stained with BARON (N-term) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-13787 BARON (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). BARON immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells. |
 | Formalin-fixed and paraffin-embedded human lymph with BARON Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of MDA-231 cells using BARON Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.