Rabbit Anti-Human BCKDHB (N-term)

Rabbit Anti-Human BCKDHB (N-term) Antibody, 400 ul
In stock

Product Description


Size 400 µl
Protein Name / Synonyms BCKDHB (N-term)
Accession Number P21953
Gene Symbols BCKDHB
Gene Id 594
Concentration (lot specific) 0.450 mg/ml
Preparation This BCKDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human BCKDHB.
Host Species Rabbit
Clonality Polyclonal
Species Human


Branched-chain keto acid dehydrogenase is a multienzyme complex associated with the inner membrane of mitochondria, and functions in the catabolism of branched-chain amino acids. The complex consists of multiple copies of 3 components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). This gene encodes the E1 beta subunit, and mutations therein have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation, and feeding problems.


Other Names
BCKDHB; 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
NCBI Accession #
Other Accession #


Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
BCKDHB Antibody (N-term) (Cat# 102-10747) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow).
BCKDHB Antibody (N-term) (Cat# 102-10747) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCKDHB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of BCKDHB (arrow) using rabbit polyclonal BCKDHB Antibody (N-term) (Cat# 102-10747). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.
BCKDHB Antibody (N-term) (Cat# 102-10747) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of BCKDHB Antibody (N-term)(Cat# 102-10747) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source



2-8°C (short-term); -20°C (long-term)
12 months from the date of shipment when stored properly.
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