beta A4 Crystallin antibody [N1C3-2], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 1413 |
| Protein Name / Synonyms | beta A4 Crystallin |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
MCOPCT4 antibody, CRYBA4 antibody, beta-crystallin A4 antibody, beta-A4 crystallin antibody, "crystallin, beta polypeptide A4 antibody", eye lens structural protein antibody, "crystallin, beta A4 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
22 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 196 of beta A4 Crystallin (Uniprot ID# P53673)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-10847 diluted at 1:1000
A: mouse brain
12% SDS PAGE
107-10847 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-crystallin A4(107-10847) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
B: HeLa
12% SDS PAGE
107-10847 diluted at 1:1000
A: H1299
B: HeLa
12% SDS PAGE
107-10847 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.