beta Tubulin antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 203068 |
| Protein Name / Synonyms | beta Tubulin |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
M40 antibody, MGC117247 antibody, MGC16435 antibody, OK/SW-cl.56 antibody, TUBB1 antibody, TUBB5 antibody, TUBB antibody, beta1-tubulin antibody, beta-4 tubulin antibody, tubulin beta-1 chain antibody, tubulin beta chain antibody, tubulin beta polypeptide antibody, "tubulin, beta polypeptide antibody", class I beta-tubulin antibody, beta Ib tubulin antibody, beta 5-tubulin antibody, tubulin beta-5 chain antibody, "tubulin, beta antibody"
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
50 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 238 and 429 of beta Tubulin
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin(107-10252) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using beta Tubulin(107-10252) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
107-10252 diluted at 1:5000
A: NIH-3T3
10% SDS PAGE
107-10252 diluted at 1:5000
Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
107-10252 diluted at 1:10000
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
107-10252 diluted at 1:10000
beta Tubulin antibody detects beta Tubulin protein at cytoplasm on breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded breast carcinoma.
beta Tubulin antibody (107-10252) dilution: 1:500.
Sample: Paraffin-embedded breast carcinoma.
beta Tubulin antibody (107-10252) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
10% SDS PAGE
107-10252 diluted at 1:3000
A: 293T
B: H1299
10% SDS PAGE
107-10252 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.