Rabbit Anti-Human Bi-Phospho-Syk(Y525/526) Antibody, 200 µl
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Product Description
Specifications
| Size | 200 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P43405 |
| Gene Id | 6850 |
| Gene Symbols | SYK |
| Protein Name / Synonyms | Bi-Phospho-Syk(Y525/526) |
| Clonality | Polyclonal |
| Preparation | This Syk Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y525/526 of human Syk. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.
Specificity
Predicted Reactivity
Mouse, Rat, Pig
Other Names
SYK; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk
NCBI Accession #
NP_001128524.1;NP_001167638.1;NP_001167639.1;NP_003168.2
Other Accession #
Q64725; Q00655; P48025
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
72066
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-Phospho-Syk Pab(Cat# 102-15362) in mouse liver tissue lysate (35ug/lane). Phospho-Syk(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.