Rabbit Anti-Human BMI1 Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P35226 |
| Gene Id | 648 |
| Gene Symbols | BMI1 |
| Protein Name / Synonyms | BMI1 |
| Clonality | Polyclonal |
| Preparation | This BMI1 antibody is generated from rabbits immunized with BMI1 recombinant protein. |
| Concentration (lot specific) | 0.280 mg/ml |
| Recommended Applications | IF, FC, Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Specificity
Other Names
BMI1; PCGF4; RNF51; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51
NCBI Accession #
NP_001190991.1;NP_005171.4
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
36949
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of BMI1 Antibody (Cat# 102-18016) in MCF7 cell line lysates (35ug/lane). BMI1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with BMI1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of BMI1 Antibody(Cat# 102-18016) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). |
 | BMI1 Antibody (Cat# 102-18016) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.