Rabbit Anti-Human CAF-1 (N-term)

Rabbit Anti-Human CAF-1 (N-term) Antibody, 400 ul
In stock

Product Description


Size 400 µl
Protein Name / Synonyms CAF-1 (N-term)
Accession Number Q9UIV1
Gene Symbols CNOT7
Gene Id 29883
Concentration (lot specific) 0.310 mg/ml
Preparation This CAF-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-61 amino acids from the N-terminal region of human CAF-1.
Host Species Rabbit
Clonality Polyclonal
Species Human


The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms.


Predicted Reactivity
Mouse, Zebrafish, Bovine, Chicken, Xenopus
Other Names
CNOT7; CAF1; CCR4-NOT transcription complex subunit 7; BTG1-binding factor 1; CCR4-associated factor 1
NCBI Accession #
Other Accession #
Q3KQ85; Q60809; Q08BM8; Q5ZJV9; Q3ZC01; NP_473367.2


Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50
CAF-1 Antibody (N-term) (Cat# 102-10944) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).
CAF-1 Antibody (N-term) (Cat# 102-10944)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term)(Cat# 102-10944).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Antigen Source



2-8°C (short-term); -20°C (long-term)
12 months from the date of shipment when stored properly.
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