Synonyms
CAP antibody, CAP1-PEN antibody, CAP1 antibody, CAP 1 antibody, adenylyl cyclase-associated protein 1 antibody, "CAP, adenylate cyclase-associated protein 1 (yeast) antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
52 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 266 and 475 of CAP1 (Uniprot ID# Q01518)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft , using CAP1(107-11033) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
B: Raji
10% SDS PAGE
107-11033 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.